Table of Contents
What is a Polymerase Chain Reaction?
A Polymerase Chain Reaction (PCR) is a technique used to amplify a specific DNA sequence. The PCR process uses two primers, which are short pieces of DNA that flank the target sequence. The primers designed to bind to the target sequence, and the polymerase enzyme then uses these primers to amplify the target DNA. The PCR process can repeated multiple times, which results in an exponential increase in the amount of amplified DNA.
About the Polymerase Chain Reaction
The polymerase chain reaction (PCR) a molecular biology technique used to amplify a single copy or a few copies of a DNA sequence. PCR is based on the ability of DNA polymerases to amplify a DNA template by copying it many times. The polymerase chain reaction developed by Kary Mullis in 1983, for which he awarded the Nobel Prize in Chemistry in 1993. PCR used in a wide variety of applications, including DNA sequencing, genetic fingerprinting, and prenatal diagnosis.
Principles of the scientific method
The scientific method is a system of acquiring knowledge that is based on empirical evidence. It involves making observations, formulating hypotheses, and testing those hypotheses through experimentation. The scientific method is a reliable way of acquiring knowledge because it based on the principle of falsifiability, which means that any hypothesis can disproven through experimentation.
A DNA Polymerase
A DNA polymerase is an enzyme that helps to create new strands of DNA. It attaches to the DNA molecule and uses the existing DNA as a template to create a new strand of DNA.
A DNA Template
A DNA Template is a molecule of DNA that can used as a model for copying DNA strands. It can also used as a tool for studying the genetic code.
PCR Process or Steps of PCR
The PCR process, also known as the Polymerase Chain Reaction, a process used to amplify a specific DNA sequence. The process uses a DNA polymerase enzyme to replicate the DNA sequence, creating many copies of the DNA. The PCR process used to amplify DNA sequences for a variety of applications, including DNA sequencing, forensics, and gene therapy.
The PCR process is a three step process. The first step is to denature the DNA, which breaks the DNA strands into individual nucleotides. The second step is to anneal the DNA primers to the DNA strands. The primers are short sequences of DNA that designed to bind to the specific DNA sequence that is to be amplified. The third step is to polymerize the DNA, which uses the DNA polymerase enzyme to create new DNA strands by combining the nucleotides.
