UncategorizedBrown Ring Test – Procedure and Applications

Brown Ring Test – Procedure and Applications

Brown Ring Test

Brown Ring Test – Procedure: The brown ring test is a test used to determine the presence of a ring of brown pigment around the margin of the iris. The test used to assess the risk of developing glaucoma, a condition in which the pressure inside the eye increases and can damage the optic nerve. The test performed by dilating the pupil and looking for a brown ring around the edge of the iris.

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    In chemistry, the brown ring test used to determine the presence of an aldehyde or ketone. This test performed by adding a few drops of a solution of Benedict’s reagent to a small sample of the substance tested. If aldehydes or ketones are present, they will react with Benedict’s reagent to form a brown ring around the edge of the sample.

    Brown Ring Test - Procedure and Applications

    Brown Ring Test

    • The brown ring test is a test used to determine the presence of blood in the stool. A small amount of stool placed on a piece of paper and also a drop of hydrogen peroxide added. If blood is present, a brown ring will form.
    • The brown ring test performed by adding a small amount of the sugar tested to a few drops of Fehling’s solution or Benedict’s solution. If a reducing sugar is present, a red-brown precipitate will form.

    Applications of Brown Ring Test

    • The Brown Ring Test is a microbiological assay that used to determine the presence of coliform bacteria in a sample. It is a qualitative test that uses a special media to detect the presence of these bacteria.
    • The media is a brown ring that forms around the colonies of coliform bacteria. However the Brown Ring Test qualitative test used to determine the presence of reducible sulfur in a hydrocarbon sample.
    • Therefore the test is based on the formation of a brown ring around the edge of a test tube containing the sample and aqueous potassium hydroxide.
    • The ring formed due to the formation of a thiosulfate complex between the sulfur and the potassium hydroxide.
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