Fehling Test
Fehling Test – Objectives: The Fehling Test is a chemical test that is used to determine the presence of reducing sugars. The test is performed by adding a drop of Benedict’s Solution to a sample of the substance being tested. If the substance contains a reducing sugar, the Benedict’s Solution will change color from blue to red.
Objectives and common uses of the Fehling Test are available for a free download at Infinity Learn
The Fehling Test used to determine the presence of reducing sugars in a solution. The test uses copper sulfate and alkali to produce a blue-green color in the presence of reducing sugars. The intensity of the color used to determine the amount of reducing sugar in the solution.
Objectives of the Fehling Test
The Fehling Test a biochemical assay used to determine the presence of reducing sugars. The assay is based on the reduction of Cu2+ to Cu+ by the reducing sugar. The Cu+ then detected by its ability to form a blue complex with the reagent Fehling A.
The Fehling Solution
The Fehling Solution a chemical test used to determine the presence of reducing sugars. The solution made up of copper sulfate and sodium hydroxide. When a reducing sugar is present, it will reduce the copper sulfate to copper metal. This will cause a blue-green color to develop in the solution.
Process of Fehling Test
The Fehling Test a simple test used to determine whether a sugar is present in a solution. A copper sulfate solution added to a sugar solution. If a sugar is present, it will react with the copper sulfate to form a copper sugar complex. The presence of this complex can easily detected by its characteristic blue color.
Reactions of Fehling’s Test
A positive reaction for the presence of aldehydes or ketones in a sample will result in a brick-red precipitate. However the presence of a sugar will not cause a reaction.
Common Uses of Fehling’s Test
One common use of Fehling’s test is to determine the presence of sugar in a substance. This is because sugar can react with the Fehling’s solution to produce a red precipitate.
Uses of Fehling Test
The Fehling Test used to determine the presence of reducing sugars in a sample. The test uses copper (II) sulfate and alkaline potassium tartrate to form a red-orange precipitation when reducing sugars are present in the sample.
Principle/Aim of the Fehling Test
The principle of the Fehling Test is that aldehydes and ketones reduce Cu2+ ions to Cu+ ions in the presence of an alkali. Therefore the presence of aldehydes or ketones in a substance can detected by the presence of a blue precipitate.
