Table of Contents
What is Chromatography?
Partition Chromatography: Chromatography is a technique used to separate the components of a mixture. The mixture is placed on a stationary phase and a mobile phase is used to move the components of the mixture through the stationary phase. The different components of the mixture will move through the stationary phase at different rates, allowing them to be separated.
What is Partition Chromatography?
Partition chromatography is a separation technique in which a mixture dissolved in a solvent and then passed through a filter. The separated components of the mixture then recovered from the filter.
Principle of Chromatography
A principle of chromatography is that different molecules in a mixture will travel through a stationary phase at different rates. This is because different molecules have different sizes, shapes, and polarities. The different molecules will also interact differently with the stationary phase.
Partition Chromatography Principle
The partition chromatography principle states that a solute will distribute itself between two immiscible liquids according to its distribution coefficient. The higher the distribution coefficient, the more the solute will partition into the more concentrated liquid.
Paper Chromatography Diagram – A type of Partition Chromatography
A type of Partition Chromatography in which the stationary phase is a sheet of paper. The mobile phase is a liquid, which drawn up the paper by capillary action. Therefore the different components of the mixture drawn up the paper at different rates, according to their relative solubilities in the two phases. This produces a series of colored bands, which can used to identify the different components of the mixture.
Types of Partition Chromatography
There are three types of partition chromatography:
Ion-exchange chromatography
Ion-exchange chromatography is a type of partition chromatography that uses a stationary phase that made up of ion-exchange resins. These resins made up of small, positively charged beads. The molecules that separated placed in a liquid solution that also mixed with a salt. The salt will bind to the small, positively charged beads on the resin. The molecules that separated will then pulled through the resin by the salt.
The molecules that pulled through the resin will separated based on how well they bind to the resin. Molecules that bind best to the resin will the most tightly attached to the resin and will the last to pulled through. The molecules that bind the least to the resin will the first to pulled through.
This type of chromatography used to separate molecules based on their charge. Therefore the most common application of this type of chromatography is the separation of proteins based on their charge.
Affinity chromatography
Affinity chromatography is a type of partition chromatography that uses a stationary phase that made up of antibodies or other proteins that have a specific affinity for a certain molecule. The molecules that separated placed in a liquid solution that also mixed with the antibody or protein. The antibody or protein will bind to the molecule
Applications of Partition Chromatography
Some possible applications of partition chromatography include the separation of proteins, carbohydrates, and lipids. Partition chromatography can also used to purify drugs and other molecules.
Partition Chromatography and How to study it?
A method of separation in which a mixture dissolved in a solvent and the components then separated by passing the mixture through a filter. The components then collected based on their different solubilities in the two phases.
