Study MaterialsNCERT SolutionsNCERT Solutions For Class 12 BiologyNCERT Solutions for class 12 Biology chapter 11 biotechnology principles and processes 11.2 tools of recombinant dna technology

NCERT Solutions for class 12 Biology chapter 11 biotechnology principles and processes 11.2 tools of recombinant dna technology

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NCERT Solutions for Class 12 Biology Chapter 11 Topic- Tools of Recombinant DNA Technology

NCERT Solutions for Class 12 Biology Chapter 11 Topic- Tools of Recombinant DNA Technology and all chapter Topics produced by qualified teachers according to NCERT (CBSE) book rules are available as a free PDF download. Class 12 Biology Chapter 11 Topic- Tools of Recombinant DNA Technology Questions with Solutions to assist you in revising the entire syllabus and achieving higher grades. Register to receive all exercise solutions through email.

Solutions

Overview of topic

The technique of recombinant DNA technology aids in the manufacture of transgenic organisms. This procedure necessitates the use of specific tools. The following are the basic processes in the DNA technology process.

(i) Genomic DNA: A donor’s genomic DNA is isolated. This DNA is used to isolate the target gene.

(ii) Vector: For the insertion of the desired gene, a vector, usually a plasmid, is utilized.

(iii) Restriction endonucleases: These enzymes are responsible for the fragmentation of DNA. The DNA is fragmented by restriction enzymes such endonucleases. Endonuclease enzymes are referred to as molecular scissors because they nick the DNA fragment at the desired location.

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    (iv) Ligase: Ligase is the enzyme that joins the two fragments together. With the help of the ligase enzyme, the desired gene is inserted into the vector and connected.

    (v) Host: A competent host cell receives the recombinant vector. The host cells are cloned or reproduced in order to produce a larger number of recombinant vectors that can be employed to make the product.

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